abstract：AIMS:Iron overload is a major factor contributing to the overall pathology of thalassaemia, which is primarily mediated by ineffective erythropoiesis and shorter mature red blood cell (RBC) survival. Iron accumulation in RBCs generates reactive oxygen species (ROS) that cause cellular damage such as lipid peroxidation ...

journal_title：Journal of clinical pathology

authors： Chaichompoo P,Qillah A,Sirankapracha P,Kaewchuchuen J,Rimthong P,Paiboonsukwong K,Fucharoen S,Svasti S,Worawichawong S

更新日期：2019-08-01 00:00:00

abstract：:Normal haemoglobin is a tetramer molecule, consisting of two α and β haemoglobin chains. Haemoglobinopathies occur when abnormalities in these proteins are present. More than 1000 naturally occurring human haemoglobin variants with single amino acid substitution throughout the molecule have been identified and can be ...

journal_title：Journal of clinical pathology

authors： Ruetsch C,Raffort J,Panaia-Ferrari P,Deconde C,Caruba-Bafghi C,Naimi M,Kavafyan J,Suissa L,Chinetti G

更新日期：2019-07-01 00:00:00

abstract：AIMS:Our laboratory is a centralised centre receiving routine non-small cell lung cancer (NSCLC) samples for programmed death ligand-1 (PD-L1) immunohistochemical (IHC) evaluation. Since literature data are not concordant here we review our clinical records to assess the rate of PD-L1 positive and negative NSCLC cases ...

journal_title：Journal of clinical pathology

authors： Vigliar E,Malapelle U,Iaccarino A,Acanfora G,Pisapia P,Clery E,De Luca C,Bellevicine C,Troncone G

更新日期：2019-06-01 00:00:00

abstract：:We determined the relative strengths of association between 23 most commonly ordered laboratory tests and the adverse outcome as indicators of relative criticalness. The lowest and highest results for 23 most commonly ordered laboratory tests, 24 hours prior to death during critical care unit (CCU) stay or discharge f...

journal_title：Journal of clinical pathology

authors： Yang Z,Tan EH,Li Y,Lim B,Metz MP,Loh TP

更新日期：2019-04-01 00:00:00

abstract：:Cutaneous melanoma causes most of the skin cancer deaths in the USA. Melanoma is the fifth most common cancer among men and the sixth most common cancer among women. The incidence of melanoma has risen sharply over the past three decades. In this review, which is informed by our extensive experience at a large cancer ...

journal_title：Journal of clinical pathology

authors： Trinidad CM,Torres-Cabala CA,Curry JL,Prieto VG,Aung PP

更新日期：2019-04-01 00:00:00

abstract：AIMS:Most benign breast fibroepithelial lesions (FEL) in adults harbour recurrent somatic MED12 exon 2 mutations and rare TERT promoter hotspot mutations. We sought to determine the frequency of MED12 exon 2 and TERT promoter hotspot mutations in fibroadenomas (FA) and benign phyllodes tumours (BePT) in adolescents and...

journal_title：Journal of clinical pathology

authors： Pareja F,Da Cruz Paula A,Murray MP,Hoang T,Gularte-Mérida R,Brown D,da Silva EM,Sebastiao APM,Giri DD,Weigelt B,Reis-Filho JS,Brogi E

更新日期：2019-03-01 00:00:00

abstract：AIMS:Clot waveform analysis (CWA) has been reported to extend the interpretation of clotting time measurement. The parameters obtained from successive derivatives of the clotting reaction curves reflect the rates of activation of individual coagulation factors, theoretically dissecting the cascade pathway. This study a...

journal_title：Journal of clinical pathology

authors： Wakui M,Fujimori Y,Katagiri H,Nakamura S,Kondo Y,Kuroda Y,Nakagawa T,Shimizu N,Murata M

更新日期：2019-03-01 00:00:00

abstract：AIMS:The JAK2 V617F mutation is highly recurrent in many of the myeloproliferative neoplasms, a molecular variant that can be easily detected using sensitive and minimally invasive techniques. Given the ease of JAK2 V617F testing, this test may be improperly requested for the purposes of patient 'screening' and to opti...

journal_title：Journal of clinical pathology

authors： Mahe E,Pedersen KM,Çolak Y,Bojesen SE,Lynch T,Sinclair G,Khan F,Shabani-Rad MT

更新日期：2019-02-01 00:00:00

abstract：:Apocrine morphology is a common phenomenon encountered in everyday breast pathology practice, and is defined as cuboidal or columnar cells exhibiting abundant eosinophilic granular cytoplasm, prominent apical granules, a low nuclear-cytoplasmic ratio, and round nuclei with pale chromatin and prominent nucleoli. Apocri...

journal_title：Journal of clinical pathology

authors： D'Arcy C,Quinn C

更新日期：2019-01-01 00:00:00

abstract：CLINICAL QUESTION:A 45-year-old woman presented with painless vaginal bleeding for 2 months. A biopsy was taken from the uterine cervix, and poorly differentiated adenocarcinoma was suspected. After three cycles of neoadjuvant chemotherapy treatment, the patient underwent radical hysterectomy with bilateral salpingo-oo...

journal_title：Journal of clinical pathology

authors： Ling C,Shen Y

更新日期：2018-11-01 00:00:00

abstract：AIM:We examined the genetic alterations in a mother and son with multiple eosinophilic chromophobe renal cell carcinomas (chRCCs) showing no other features. METHODS:Germline DNA and bilateral renal cell carcinoma DNA were genetically analysed by whole-exome sequencing. Candidate gene alterations in the first patient's...

journal_title：Journal of clinical pathology

authors： Sakamoto H,Yamasaki T,Sumiyoshi T,Utsunomiya N,Takeda M,Kamba T,Nakamura E,Ogawa O

更新日期：2018-10-01 00:00:00

abstract：AIMS:Multiple myeloma (MM) is a heterogeneous disease characterised by genetically complex abnormalities. The classical mutational spectrum includes recurrent chromosomal aberrations and gene-level mutations. Recurrent translocations involving the IGH gene such as t(11;14), t(4;14) and t(14;16) are well known. However,...

journal_title：Journal of clinical pathology

authors： Lin M,Lee PL,Chiu L,Chua C,Ban KHK,Lin AHF,Chan ZL,Chung TH,Yan B,Chng WJ

更新日期：2018-08-01 00:00:00

abstract：AIMS:Dysfunctional prothrombin residue Arg596 associated mutation has been found to precipitate venous thromboembolism (VTE). In the current study we investigated the prevalence of Arg596 associated mutations in Chinese patients with VTE and explored the functional impact of Arg596Gln mutation on coagulation function i...

journal_title：Journal of clinical pathology

authors： Wu X,Li L,Ding Q,Wang X,Wu F,Wu W

更新日期：2018-07-01 00:00:00

abstract：AIMS:N-terminal pro-B-type natriuretic peptide (NT-proBNP) has been shown to be useful for ruling out heart failure in primary care. In this study, we examined the accuracy of the Cobas h232 point-of-care (POC) instrument in primary care compared with an in-hospital measurement. Furthermore, we investigated the user-fr...

journal_title：Journal of clinical pathology

authors： Hex C,Smeets M,Penders J,Van Hoof V,Verbakel J,Buntinx F,Vaes B

更新日期：2018-06-01 00:00:00

abstract：AIMS:This study was aimed to investigate the role of erythrocyte, platelet and reticulocyte (RET) parameters, measured by new haematological analyser Sysmex XN and C reactive protein (CRP), for early diagnosis of sepsis during intensive care unit (ICU) stay. METHODS:The study population consisted of 62 ICU patients, 2...

journal_title：Journal of clinical pathology

authors： Buoro S,Manenti B,Seghezzi M,Dominoni P,Barbui T,Ghirardi A,Carobbio A,Marchesi G,Riva I,Nasi A,Ottomano C,Lippi G

更新日期：2018-04-01 00:00:00

abstract：:Programmed death ligand 1 (PD-L1) is the principal ligand of programmed death 1 (PD-1), a coinhibitory receptor that can be constitutively expressed or induced in myeloid, lymphoid, normal epithelial cells and in cancer. Under physiological conditions, the PD-1/PD-L1 interaction is essential in the development of immu...

journal_title：Journal of clinical pathology

authors： Kythreotou A,Siddique A,Mauri FA,Bower M,Pinato DJ

更新日期：2018-03-01 00:00:00

abstract：AIMS:To identify calcifying epithelial odontogenic tumour (CEOT) mutations in oncogenes and tumour suppressor genes. METHODS:A panel of 50 genes commonly mutated in cancer was sequenced in CEOT by next-generation sequencing. Sanger sequencing was used to cover the region of the frameshift deletion identified in one sa...

journal_title：Journal of clinical pathology

authors： de Sousa SF,Diniz MG,França JA,Fontes Pereira TDS,Moreira RG,Santos JND,Gomez RS,Gomes CC

更新日期：2018-03-01 00:00:00

abstract：BACKGROUND:Triple-negative breast cancers (TNBCs) are defined by their lack of oestrogen receptor, progesterone receptor and epidermal growth factor receptor 2. Although heterogeneous, the majority are aggressive and treatment options are limited. Caveolin acts as tumour suppressor or promoter depending on the cancer t...

journal_title：Journal of clinical pathology

authors： Yeong J,Thike AA,Ikeda M,Lim JCT,Lee B,Nakamura S,Iqbal J,Tan PH

更新日期：2018-02-01 00:00:00

abstract：AIMS:To validate the Singapore nomogram for outcome prediction in breast phyllodes tumours in a large cohort of Singaporean patients, as previous validation studies were conducted on small numbers of patients. We also investigate the association of fibroadenomas and phyllodes tumours within a subset of our cohort. MET...

journal_title：Journal of clinical pathology

authors： Chng TW,Gudi M,Lim SH,Li H,Tan PH

更新日期：2018-02-01 00:00:00

abstract：AIMS:The probabilistic approach is widely adopted for breast fine needle aspiration cytology. However, a definite cytological diagnosis is not always possible for C3 (atypia) cases, which poses a management dilemma as this represents a mixed category of benign and malignant cases. It would be beneficial to be able to p...

journal_title：Journal of clinical pathology

authors： Yu SN,Li J,Wong SI,Tsang JYS,Ni YB,Chen J,Tse GM

更新日期：2017-12-01 00:00:00

abstract：AIMS:The aim of this study was to develop a computer-aided diagnosis (CADx) system for identifying breast pathology. METHODS:Two sets of 100 consecutive core needle biopsy (CNB) specimens were collected for test and validation studies. All 200 CNB specimens were stained with antibodies targeting oestrogen receptor (ER...

journal_title：Journal of clinical pathology

authors： Maeda I,Kubota M,Ohta J,Shinno K,Tajima S,Ariizumi Y,Doi M,Oana Y,Kanemaki Y,Tsugawa K,Ueno T,Takagi M

更新日期：2017-12-01 00:00:00

abstract：AIM:Nowadays, extracellular vesicles are of great interest in prostate cancer (PCa) research. Asparagine (N)-linked glycosylation could play a significant role in the pathological mechanism of these vesicles. We investigated if prostatic protein N-glycosylation profiles were related to urinary vesicle-associated prosta...

journal_title：Journal of clinical pathology

authors： Vermassen T,D'Herde K,Jacobus D,Van Praet C,Poelaert F,Lumen N,Callewaert N,Decaestecker K,Villeirs G,Hoebeke P,Van Belle S,Rottey S,Delanghe J

更新日期：2017-10-01 00:00:00

abstract：:Pre-mRNA splicing is an essential process in eukaryotic cells where the transcribed intronic sequences are removed, prior to translation into protein. PRPF31 is a ubiquitously expressed splicing factor, which aids in the assembly of the macromolecular spliceosome. Mutations in PRPF31 cause autosomal dominant retinitis...

journal_title：Journal of clinical pathology

authors： Rose AM,Luo R,Radia UK,Bhattacharya SS

更新日期：2017-09-01 00:00:00

abstract：:Microsatellite instability (MSI) testing is tricky in gynaecological cancers (GC). Thus, we aimed to describe the instability patterns to improve MSI test interpretation in sporadic and hereditary GCs. Ninety-five cases, including uterine and ovarian cancers, with known genetic and immunohistochemical (IHC) features, ...

journal_title：Journal of clinical pathology

authors： Libera L,Sahnane N,Carnevali IW,Cimetti L,Cerutti R,Chiaravalli AM,Riva C,Tibiletti MG,Sessa F,Furlan D

更新日期：2017-09-01 00:00:00

abstract：AIMS:Ki-67 is a prognostic marker in breast cancer; however, the use of the Ki-67 labelling index (LI) in clinical practice requires a consistent and easily accessible scoring method. The present study evaluated the use of the free internet-based image analysis program ImmunoRatio to score Ki-67 LI in breast cancer in ...

journal_title：Journal of clinical pathology

authors： Yeo MK,Kim HE,Kim SH,Chae BJ,Song BJ,Lee A

更新日期：2017-08-01 00:00:00

abstract：CLINICAL QUESTION:A 77-year-old man presented with haematuria. Cystoscopy revealed a papillary tumour in the trigone region of the bladder. A TURBT was performed. Review the high-quality, interactive digital Aperio slide at http://virtualacp.com/JCPCases/jclinpath-2016-204015 and consider your diagnosis. WHAT IS YOUR D...

journal_title：Journal of clinical pathology

authors： Trefor R,Varma M

更新日期：2017-07-01 00:00:00

abstract：AIMS:Primary cilia play an important role in the regulation of cell signalling pathways and are thought to have a role in cancer but have seldom been studied in human cancer samples. METHODS:Primary cilia were visualised by dual immunofluorescence for anti-CROCC (ciliary rootlet coiled-coil) and anti-tubulin in a rang...

journal_title：Journal of clinical pathology

authors： Yasar B,Linton K,Slater C,Byers R

更新日期：2017-07-01 00:00:00

abstract：AIM:IgG4 disease is rare. However, IgG4 tubulointerstitial nephritis (TIN) is the most common renal manifestation. IgG4 disease is usually associated with elevated serum IgG4 levels and other organ involvement, low-density renal lesions on enhanced CT imaging and immune activation. The incidence of IgG4-TIN may be unde...

journal_title：Journal of clinical pathology

authors： Mac K,Wu XJ,Mai J,Howlin K,Suranyi M,Yong J,Makris A

更新日期：2017-06-01 00:00:00

abstract：:Haematological malignancies are a diverse group of cancers that affect the blood, bone marrow and lymphatic systems. Laboratory diagnosis of haematological malignancies is dependent on combining several technologies, including morphology, immunophenotyping, cytogenetics and molecular genetics correlated clinical detai...

journal_title：Journal of clinical pathology

authors： Snowden JA,O'Connell S,Hawkins J,Dalley C,Jack A,Mannari D,McNamara C,Scott M,Shenton G,Soilleux E,Macbeth F,Guideline Committee.

更新日期：2017-06-01 00:00:00

abstract：OBJECTIVES:As the best characterised cancer/testis antigen family members, melanoma-associated antigens (MAGE) have been reported to be expressed in various malignant tumours. However, the expression pattern of MAGE-A family in lung adenocarcinoma (LAC) specimens and their prognostic and therapeutic significance for pa...

journal_title：Journal of clinical pathology

authors： Sang M,Gu L,Yin D,Liu F,Lian Y,Zhang X,Liu S,Huang W,Wu Y,Shan B

更新日期：2017-06-01 00:00:00

abstract：AIMS:Determine whether a simple prewash step will provide adequate amounts of high-quality DNA from core needle biopsies for molecular sequencing studies. METHODS:The quantitative and qualitative metrics of DNA recovered from core needle biopsies processed either by 1) formalin fixation and paraffin embedding (FFPE), ...

journal_title：Journal of clinical pathology

authors： Mojica WD,Hou T,Sykes D,Dey-Rao R

更新日期：2017-06-01 00:00:00

abstract：AIMS:Computational pathology platforms incorporate digital microscopy with sophisticated image analysis to permit rapid, continuous measurement of protein expression. We compared two computational pathology platforms on their measurement of breast tumour oestrogen receptor (ER) and progesterone receptor (PR) expression...

journal_title：Journal of clinical pathology

authors： Ahern TP,Beck AH,Rosner BA,Glass B,Frieling G,Collins LC,Tamimi RM

更新日期：2017-05-01 00:00:00

abstract：:Digital pathology (DP) and whole-slide imaging (WSI) technology have matured substantially over the last few years and there is growing evidence from validation studies that WSI is comparable to glass slides for histopathology diagnosis, although with some limitations, which can be appropriately minimised. Whether the...

journal_title：Journal of clinical pathology

authors： Cheng CL,Tan PH

更新日期：2017-05-01 00:00:00

abstract：AIMS:In everyday practice, epidermal growth factor receptor (EGFR) testing is centralised in referral laboratories that receive paucicellular cytological specimens. Ideally, EGFR testing should be carried out in the centre where the patient is diagnosed such that the most cellular slide can be selected from in-house co...

journal_title：Journal of clinical pathology

authors： De Luca C,Gragnano G,Pisapia P,Vigliar E,Malapelle U,Bellevicine C,Troncone G

更新日期：2017-04-01 00:00:00

abstract：AIMS:Acute traumatic coagulopathy is characterised by fibrinolysis and low fibrinogen. It is unclear how much fibrinogenolysis contributes to reduce fibrinogen levels. The study aim was to: investigate in vitro the effects of tissue-plasminogen activator (t-PA) and tranexamic acid (TXA) on coagulation and fibrinolysis....

journal_title：Journal of clinical pathology

authors： Godier A,Parmar K,Manandhar K,Hunt BJ

更新日期：2017-02-01 00:00:00

abstract：OBJECTIVE:Acute B lymphoblastic leukaemia (B-ALL) is the most common type of childhood malignancy worldwide but little is known of its origin. Recently, many studies showed both a high incidence of Epstein-Barr virus (EBV) infection and high levels of CD4+CD25+Foxp3+(Treg cells) in children with B-ALL. In our study, we...

journal_title：Journal of clinical pathology

authors： Ateyah ME,Hashem ME,Abdelsalam M

更新日期：2017-02-01 00:00:00

abstract：AIMS:Sentinel lymph node (SLN) biopsy is the preferred surgical technique for staging the axilla in clinically node-negative breast cancer. Accurate intraoperative staging allows for the immediate performance of an axillary clearance in node-positive patients. We assessed the Metasin assay for the intraoperative analys...

journal_title：Journal of clinical pathology

authors： Smith GJ,Hodges E,Markham H,Zhang S,Cutress RI

更新日期：2017-02-01 00:00:00

abstract：AIM:Heart failure (HF) affects millions of older individuals in both developed and low/middle-income countries. Serum galectin-3 levels have been shown to have prognostic value. However, its use as a diagnostic biomarker has not been explored. The aim was to establish a saliva galectin-3 reference range and to demonstr...

journal_title：Journal of clinical pathology

authors： Zhang X,Wan Y,Chata R,Brazzale A,Atherton JJ,Kostner K,Dimeski G,Punyadeera C

更新日期：2016-12-01 00:00:00

abstract：:Although the UK National Institute for Health and Care Excellence guidelines recommend that in patients with biopsy-proven invasive lobular carcinoma (ILC), preoperative MRI scan is considered, the accuracy of diagnosis of ILC in core biopsy of the breast has not been previously investigated. Eleven pathology laborato...

journal_title：Journal of clinical pathology

authors： Naidoo K,Beardsley B,Carder PJ,Deb R,Fish D,Girling A,Hales S,Howe M,Wastall LM,Lane S,Lee AH,Philippidou M,Quinn C,Stephenson T,Pinder SE

更新日期：2016-12-01 00:00:00

abstract：AIMS:The WHO classification describes that combined hepatocellular-cholangiocarcinoma, subtypes with stem-cell features, intermediate-cell subtype (CHC-INT) is composed of tumour cells with features intermediate between hepatocytes and cholangiocytes. However, we previously reported that CHC-INT showed a high positive ...

journal_title：Journal of clinical pathology

authors： Akiba J,Nakashima O,Hattori S,Naito Y,Kusano H,Kondo R,Nakayama M,Tanikawa K,Todoroki K,Umeno Y,Nakamura K,Sanada S,Yamaguchi R,Ogasawara S,Yano H

更新日期：2016-10-01 00:00:00